Palabras clave
Rhizoctonia solani
proteínas de resistencia
2-D PAGE rice
Rhizoctonia solani
resistance proteins
2-D PAGE
Cómo citar
Resumen
El tizón de la vaina causado por Rhizotonia solani se describe como la segunda enfermedad importante que afecta al arroz. La resistencia genética a R. solani, la medida de control ideal, se ve obstaculizada debido a la dificultad de identificar fuentes de resistencia adecuadas en condiciones típicas de selección. Las técnicas de análisis proteómico que utilizan geles bidimensionales (2D-PAGE) permiten el estudio o seguimiento de cambios globales en la expresión de proteínas en condiciones normales y de estrés. En este trabajo, comparamos los patrones de expresión de proteínas en hojas de arroz de dos variedades venezolanas 12, 24 y 48 h después de la inoculación con R. solani. Aproximadamente 400 y 300 manchas de proteína teñidas con Sypro Ruby se resolvieron de forma reproducible a través de réplicas de gel, para PALMAR y FONAIAP-2000, respectivamente. Se identificaron cuarenta proteínas de un total de 49 para la variedad PALMAR, con treinta y dos manchas proteicas reguladas al alza y 8 reguladas a la baja. Se identificaron 26 proteínas de un total de 33 para la variedad FONAIAP-2000, con siete manchas proteicas reguladas al alza y 19 reguladas a la baja. RuBisCo fue la proteína más identificada (48% y 82% de las proteínas detectadas para PALMAR y FONAIAP-2000, respectivamente). Otras proteínas identificadas que mostraron variaciones fueron la subunidad beta de la ATPasa, la UDP-glucosa antocianina 5-O-glucosiltransferasa, la proteína de unión al ARN, la supuesta transkelotasa 1, la supuesta ferredoxina-NAPD (H) óxido-reductasa y el supuesto fotosistema II de la proteína que evoluciona el oxígeno de 33 kDa. Con base en nuestros resultados, la respuesta del arroz a R. solani podría describirse donde se requiere energía para inducir una defensa y es suministrada por proteínas involucradas en el metabolismo energético. Según esto, proteomic podría proporcionar información y conocimientos sobre la respuesta del arroz al desafío con R. solani y otros patógenos.
Citas
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Derechos de autor 2021 Fuchs Morela Delgado, Marisol Fernández, Iris Pérez-Almeida